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Premier Biosoft primer premier version 5.0. software
Primer Premier Version 5.0. Software, supplied by Premier Biosoft, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primer+software+version+5%2E0/primer+premier+5+0+software/pmc12206813-70-10-15
Average 90 stars, based on 1 article reviews
primer premier version 5.0. software - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Characterization and transcriptomic analysis of a novel yellow-green leaf wucai ( Brassica campestris L.) germplasm
Article Snippet: First-strand cDNA was synthesized using the PrimeScriptTM RT reagent kit (TaKaRa). qRT-PCR was performed in a 20-μL reaction volume containing 1 μL of 100 ng cDNA, 1 μL each of 10 μM forward and reverse primers, 10 μL SYBR Premix Ex Taq II (Takara), and filled with 7 μL ddH 2 O. Gene-specific primers were designed using Primer Software version 5.0 (Premier Biosoft International, CA, USA).

Article Title: The effect of exogenous 24‐epibrassinolide pretreatment on the quality, antioxidant capacity, and postharvest life of wucai ( Brassica campestris L.)
Article Snippet: The gene‐specific primers were designed using Primer software version 5.0 (Premier Biosoft International) for the qRT‐PCR (Table ) of the genes coding for APX, AAO, DHAR, and MDHAR.

Article Title: Comparative Proteomics Reveals Cold Acclimation Machinery Through Enhanced Carbohydrate and Amino Acid Metabolism in Wucai ( Brassica Campestris L.)
Article Snippet: Specific primers for differentially expressed protein-related genes were designed by Primer software version 5.0 (Premier Biosoft International, Palo Alto, CA, USA) ( ).

Article Title: Comparative Proteomics Indicates That Redox Homeostasis Is Involved in High- and Low-Temperature Stress Tolerance in a Novel Wucai ( Brassica campestris L.) Genotype
Article Snippet: Primer software version 5.0 (Premier Biosoft International, CA, USA) was used to design gene-specific primers, and primer sequences are indicated in . qRT-PCR analysis was performed using AceQ qPCR SYBR GREEN Master Mix (Vazyme Biotechnology Co., Nanjing, China), and relative expression levels were calculated using the 2 − △△ C t method [ ].

Article Title: Mdr3 gene mutation in preterm infants with parenteral nutrition‐associated cholestasis
Article Snippet: Primer Software Version 5.0 (Premier Biosoft International) was used to design the primers, and the DNA concentration was quantified using a UV–VIS spectrophotometer (UV‐5200, Shanghai Metash Instrument Co., Ltd.).

Article Title: Cold acclimation can specifically inhibit chlorophyll biosynthesis in young leaves of Pakchoi
Article Snippet: Primers for qPCR analysis were designed by Primer Software Version 5.0 (Premier Biosoft International, CA, USA) and shown in Table S .

Article Title: Comparative Proteomic Analysis Reveals That Chlorophyll Metabolism Contributes to Leaf Color Changes in Wucai ( Brassica campestris L.) Responding to Cold Acclimation.
Article Snippet: Subscriber access provided by OCCIDENTAL COLL is published by the American Chemical Society.. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society.. Copyright © American Chemical Society.

Article Title: Comparative Proteomics Reveals Cold Acclimation Machinery Through Enhanced Carbohydrate and Amino Acid Metabolism in Wucai ( Brassica Campestris L.).
Article Snippet: Specific primers for differentially expressed protein-related genes were designed by Primer software version 5.0 (Premier Biosoft International, Palo Alto, CA, USA) (Table 3).

Concentration Assay:

Article Title: Characterization and transcriptomic analysis of a novel yellow-green leaf wucai ( Brassica campestris L.) germplasm
Article Snippet: First-strand cDNA was synthesized using the PrimeScriptTM RT reagent kit (TaKaRa). qRT-PCR was performed in a 20-μL reaction volume containing 1 μL of 100 ng cDNA, 1 μL each of 10 μM forward and reverse primers, 10 μL SYBR Premix Ex Taq II (Takara), and filled with 7 μL ddH 2 O. Gene-specific primers were designed using Primer Software version 5.0 (Premier Biosoft International, CA, USA).

Article Title: The effect of exogenous 24‐epibrassinolide pretreatment on the quality, antioxidant capacity, and postharvest life of wucai ( Brassica campestris L.)
Article Snippet: The gene‐specific primers were designed using Primer software version 5.0 (Premier Biosoft International) for the qRT‐PCR (Table ) of the genes coding for APX, AAO, DHAR, and MDHAR.

Article Title: Comparative Proteomics Reveals Cold Acclimation Machinery Through Enhanced Carbohydrate and Amino Acid Metabolism in Wucai ( Brassica Campestris L.)
Article Snippet: Specific primers for differentially expressed protein-related genes were designed by Primer software version 5.0 (Premier Biosoft International, Palo Alto, CA, USA) ( ).

Article Title: Comparative Proteomics Indicates That Redox Homeostasis Is Involved in High- and Low-Temperature Stress Tolerance in a Novel Wucai ( Brassica campestris L.) Genotype
Article Snippet: Primer software version 5.0 (Premier Biosoft International, CA, USA) was used to design gene-specific primers, and primer sequences are indicated in . qRT-PCR analysis was performed using AceQ qPCR SYBR GREEN Master Mix (Vazyme Biotechnology Co., Nanjing, China), and relative expression levels were calculated using the 2 − △△ C t method [ ].

Article Title: Mdr3 gene mutation in preterm infants with parenteral nutrition‐associated cholestasis
Article Snippet: Primer Software Version 5.0 (Premier Biosoft International) was used to design the primers, and the DNA concentration was quantified using a UV–VIS spectrophotometer (UV‐5200, Shanghai Metash Instrument Co., Ltd.).

Article Title: Cold acclimation can specifically inhibit chlorophyll biosynthesis in young leaves of Pakchoi
Article Snippet: Primers for qPCR analysis were designed by Primer Software Version 5.0 (Premier Biosoft International, CA, USA) and shown in Table S .

Article Title: Comparative Proteomic Analysis Reveals That Chlorophyll Metabolism Contributes to Leaf Color Changes in Wucai ( Brassica campestris L.) Responding to Cold Acclimation.
Article Snippet: Subscriber access provided by OCCIDENTAL COLL is published by the American Chemical Society.. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society.. Copyright © American Chemical Society.

Article Title: Comparative Proteomics Reveals Cold Acclimation Machinery Through Enhanced Carbohydrate and Amino Acid Metabolism in Wucai ( Brassica Campestris L.).
Article Snippet: Specific primers for differentially expressed protein-related genes were designed by Primer software version 5.0 (Premier Biosoft International, Palo Alto, CA, USA) (Table 3).

Spectrophotometry:

Article Title: Characterization and transcriptomic analysis of a novel yellow-green leaf wucai ( Brassica campestris L.) germplasm
Article Snippet: First-strand cDNA was synthesized using the PrimeScriptTM RT reagent kit (TaKaRa). qRT-PCR was performed in a 20-μL reaction volume containing 1 μL of 100 ng cDNA, 1 μL each of 10 μM forward and reverse primers, 10 μL SYBR Premix Ex Taq II (Takara), and filled with 7 μL ddH 2 O. Gene-specific primers were designed using Primer Software version 5.0 (Premier Biosoft International, CA, USA).

Article Title: The effect of exogenous 24‐epibrassinolide pretreatment on the quality, antioxidant capacity, and postharvest life of wucai ( Brassica campestris L.)
Article Snippet: The gene‐specific primers were designed using Primer software version 5.0 (Premier Biosoft International) for the qRT‐PCR (Table ) of the genes coding for APX, AAO, DHAR, and MDHAR.

Article Title: Comparative Proteomics Reveals Cold Acclimation Machinery Through Enhanced Carbohydrate and Amino Acid Metabolism in Wucai ( Brassica Campestris L.)
Article Snippet: Specific primers for differentially expressed protein-related genes were designed by Primer software version 5.0 (Premier Biosoft International, Palo Alto, CA, USA) ( ).

Article Title: Comparative Proteomics Indicates That Redox Homeostasis Is Involved in High- and Low-Temperature Stress Tolerance in a Novel Wucai ( Brassica campestris L.) Genotype
Article Snippet: Primer software version 5.0 (Premier Biosoft International, CA, USA) was used to design gene-specific primers, and primer sequences are indicated in . qRT-PCR analysis was performed using AceQ qPCR SYBR GREEN Master Mix (Vazyme Biotechnology Co., Nanjing, China), and relative expression levels were calculated using the 2 − △△ C t method [ ].

Article Title: Mdr3 gene mutation in preterm infants with parenteral nutrition‐associated cholestasis
Article Snippet: Primer Software Version 5.0 (Premier Biosoft International) was used to design the primers, and the DNA concentration was quantified using a UV–VIS spectrophotometer (UV‐5200, Shanghai Metash Instrument Co., Ltd.).

Article Title: Cold acclimation can specifically inhibit chlorophyll biosynthesis in young leaves of Pakchoi
Article Snippet: Primers for qPCR analysis were designed by Primer Software Version 5.0 (Premier Biosoft International, CA, USA) and shown in Table S .

Article Title: Comparative Proteomic Analysis Reveals That Chlorophyll Metabolism Contributes to Leaf Color Changes in Wucai ( Brassica campestris L.) Responding to Cold Acclimation.
Article Snippet: Subscriber access provided by OCCIDENTAL COLL is published by the American Chemical Society.. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society.. Copyright © American Chemical Society.

Article Title: Comparative Proteomics Reveals Cold Acclimation Machinery Through Enhanced Carbohydrate and Amino Acid Metabolism in Wucai ( Brassica Campestris L.).
Article Snippet: Specific primers for differentially expressed protein-related genes were designed by Primer software version 5.0 (Premier Biosoft International, Palo Alto, CA, USA) (Table 3).

Synthesized:

Article Title: Characterization and transcriptomic analysis of a novel yellow-green leaf wucai ( Brassica campestris L.) germplasm
Article Snippet: First-strand cDNA was synthesized using the PrimeScriptTM RT reagent kit (TaKaRa). qRT-PCR was performed in a 20-μL reaction volume containing 1 μL of 100 ng cDNA, 1 μL each of 10 μM forward and reverse primers, 10 μL SYBR Premix Ex Taq II (Takara), and filled with 7 μL ddH 2 O. Gene-specific primers were designed using Primer Software version 5.0 (Premier Biosoft International, CA, USA).

Article Title: The effect of exogenous 24‐epibrassinolide pretreatment on the quality, antioxidant capacity, and postharvest life of wucai ( Brassica campestris L.)
Article Snippet: The gene‐specific primers were designed using Primer software version 5.0 (Premier Biosoft International) for the qRT‐PCR (Table ) of the genes coding for APX, AAO, DHAR, and MDHAR.

Article Title: Comparative Proteomics Reveals Cold Acclimation Machinery Through Enhanced Carbohydrate and Amino Acid Metabolism in Wucai ( Brassica Campestris L.)
Article Snippet: Specific primers for differentially expressed protein-related genes were designed by Primer software version 5.0 (Premier Biosoft International, Palo Alto, CA, USA) ( ).

Article Title: Comparative Proteomics Indicates That Redox Homeostasis Is Involved in High- and Low-Temperature Stress Tolerance in a Novel Wucai ( Brassica campestris L.) Genotype
Article Snippet: Primer software version 5.0 (Premier Biosoft International, CA, USA) was used to design gene-specific primers, and primer sequences are indicated in . qRT-PCR analysis was performed using AceQ qPCR SYBR GREEN Master Mix (Vazyme Biotechnology Co., Nanjing, China), and relative expression levels were calculated using the 2 − △△ C t method [ ].

Article Title: Mdr3 gene mutation in preterm infants with parenteral nutrition‐associated cholestasis
Article Snippet: Primer Software Version 5.0 (Premier Biosoft International) was used to design the primers, and the DNA concentration was quantified using a UV–VIS spectrophotometer (UV‐5200, Shanghai Metash Instrument Co., Ltd.).

Article Title: Cold acclimation can specifically inhibit chlorophyll biosynthesis in young leaves of Pakchoi
Article Snippet: Primers for qPCR analysis were designed by Primer Software Version 5.0 (Premier Biosoft International, CA, USA) and shown in Table S .

Article Title: Comparative Proteomic Analysis Reveals That Chlorophyll Metabolism Contributes to Leaf Color Changes in Wucai ( Brassica campestris L.) Responding to Cold Acclimation.
Article Snippet: Subscriber access provided by OCCIDENTAL COLL is published by the American Chemical Society.. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society.. Copyright © American Chemical Society.

Article Title: Comparative Proteomics Reveals Cold Acclimation Machinery Through Enhanced Carbohydrate and Amino Acid Metabolism in Wucai ( Brassica Campestris L.).
Article Snippet: Specific primers for differentially expressed protein-related genes were designed by Primer software version 5.0 (Premier Biosoft International, Palo Alto, CA, USA) (Table 3).

Quantitative RT-PCR:

Article Title: Characterization and transcriptomic analysis of a novel yellow-green leaf wucai ( Brassica campestris L.) germplasm
Article Snippet: First-strand cDNA was synthesized using the PrimeScriptTM RT reagent kit (TaKaRa). qRT-PCR was performed in a 20-μL reaction volume containing 1 μL of 100 ng cDNA, 1 μL each of 10 μM forward and reverse primers, 10 μL SYBR Premix Ex Taq II (Takara), and filled with 7 μL ddH 2 O. Gene-specific primers were designed using Primer Software version 5.0 (Premier Biosoft International, CA, USA).

Article Title: The effect of exogenous 24‐epibrassinolide pretreatment on the quality, antioxidant capacity, and postharvest life of wucai ( Brassica campestris L.)
Article Snippet: The gene‐specific primers were designed using Primer software version 5.0 (Premier Biosoft International) for the qRT‐PCR (Table ) of the genes coding for APX, AAO, DHAR, and MDHAR.

Article Title: Comparative Proteomics Reveals Cold Acclimation Machinery Through Enhanced Carbohydrate and Amino Acid Metabolism in Wucai ( Brassica Campestris L.)
Article Snippet: Specific primers for differentially expressed protein-related genes were designed by Primer software version 5.0 (Premier Biosoft International, Palo Alto, CA, USA) ( ).

Article Title: Comparative Proteomics Indicates That Redox Homeostasis Is Involved in High- and Low-Temperature Stress Tolerance in a Novel Wucai ( Brassica campestris L.) Genotype
Article Snippet: Primer software version 5.0 (Premier Biosoft International, CA, USA) was used to design gene-specific primers, and primer sequences are indicated in . qRT-PCR analysis was performed using AceQ qPCR SYBR GREEN Master Mix (Vazyme Biotechnology Co., Nanjing, China), and relative expression levels were calculated using the 2 − △△ C t method [ ].

Article Title: Mdr3 gene mutation in preterm infants with parenteral nutrition‐associated cholestasis
Article Snippet: Primer Software Version 5.0 (Premier Biosoft International) was used to design the primers, and the DNA concentration was quantified using a UV–VIS spectrophotometer (UV‐5200, Shanghai Metash Instrument Co., Ltd.).

Article Title: Cold acclimation can specifically inhibit chlorophyll biosynthesis in young leaves of Pakchoi
Article Snippet: Primers for qPCR analysis were designed by Primer Software Version 5.0 (Premier Biosoft International, CA, USA) and shown in Table S .

Article Title: Comparative Proteomic Analysis Reveals That Chlorophyll Metabolism Contributes to Leaf Color Changes in Wucai ( Brassica campestris L.) Responding to Cold Acclimation.
Article Snippet: Subscriber access provided by OCCIDENTAL COLL is published by the American Chemical Society.. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society.. Copyright © American Chemical Society.

Article Title: Comparative Proteomics Reveals Cold Acclimation Machinery Through Enhanced Carbohydrate and Amino Acid Metabolism in Wucai ( Brassica Campestris L.).
Article Snippet: Specific primers for differentially expressed protein-related genes were designed by Primer software version 5.0 (Premier Biosoft International, Palo Alto, CA, USA) (Table 3).

Real-time Polymerase Chain Reaction:

Article Title: Characterization and transcriptomic analysis of a novel yellow-green leaf wucai ( Brassica campestris L.) germplasm
Article Snippet: First-strand cDNA was synthesized using the PrimeScriptTM RT reagent kit (TaKaRa). qRT-PCR was performed in a 20-μL reaction volume containing 1 μL of 100 ng cDNA, 1 μL each of 10 μM forward and reverse primers, 10 μL SYBR Premix Ex Taq II (Takara), and filled with 7 μL ddH 2 O. Gene-specific primers were designed using Primer Software version 5.0 (Premier Biosoft International, CA, USA).

Article Title: The effect of exogenous 24‐epibrassinolide pretreatment on the quality, antioxidant capacity, and postharvest life of wucai ( Brassica campestris L.)
Article Snippet: The gene‐specific primers were designed using Primer software version 5.0 (Premier Biosoft International) for the qRT‐PCR (Table ) of the genes coding for APX, AAO, DHAR, and MDHAR.

Article Title: Comparative Proteomics Reveals Cold Acclimation Machinery Through Enhanced Carbohydrate and Amino Acid Metabolism in Wucai ( Brassica Campestris L.)
Article Snippet: Specific primers for differentially expressed protein-related genes were designed by Primer software version 5.0 (Premier Biosoft International, Palo Alto, CA, USA) ( ).

Article Title: Comparative Proteomics Indicates That Redox Homeostasis Is Involved in High- and Low-Temperature Stress Tolerance in a Novel Wucai ( Brassica campestris L.) Genotype
Article Snippet: Primer software version 5.0 (Premier Biosoft International, CA, USA) was used to design gene-specific primers, and primer sequences are indicated in . qRT-PCR analysis was performed using AceQ qPCR SYBR GREEN Master Mix (Vazyme Biotechnology Co., Nanjing, China), and relative expression levels were calculated using the 2 − △△ C t method [ ].

Article Title: Mdr3 gene mutation in preterm infants with parenteral nutrition‐associated cholestasis
Article Snippet: Primer Software Version 5.0 (Premier Biosoft International) was used to design the primers, and the DNA concentration was quantified using a UV–VIS spectrophotometer (UV‐5200, Shanghai Metash Instrument Co., Ltd.).

Article Title: Cold acclimation can specifically inhibit chlorophyll biosynthesis in young leaves of Pakchoi
Article Snippet: Primers for qPCR analysis were designed by Primer Software Version 5.0 (Premier Biosoft International, CA, USA) and shown in Table S .

Article Title: Comparative Proteomic Analysis Reveals That Chlorophyll Metabolism Contributes to Leaf Color Changes in Wucai ( Brassica campestris L.) Responding to Cold Acclimation.
Article Snippet: Subscriber access provided by OCCIDENTAL COLL is published by the American Chemical Society.. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society.. Copyright © American Chemical Society.

Article Title: Comparative Proteomics Reveals Cold Acclimation Machinery Through Enhanced Carbohydrate and Amino Acid Metabolism in Wucai ( Brassica Campestris L.).
Article Snippet: Specific primers for differentially expressed protein-related genes were designed by Primer software version 5.0 (Premier Biosoft International, Palo Alto, CA, USA) (Table 3).

SYBR Green Assay:

Article Title: Characterization and transcriptomic analysis of a novel yellow-green leaf wucai ( Brassica campestris L.) germplasm
Article Snippet: First-strand cDNA was synthesized using the PrimeScriptTM RT reagent kit (TaKaRa). qRT-PCR was performed in a 20-μL reaction volume containing 1 μL of 100 ng cDNA, 1 μL each of 10 μM forward and reverse primers, 10 μL SYBR Premix Ex Taq II (Takara), and filled with 7 μL ddH 2 O. Gene-specific primers were designed using Primer Software version 5.0 (Premier Biosoft International, CA, USA).

Article Title: The effect of exogenous 24‐epibrassinolide pretreatment on the quality, antioxidant capacity, and postharvest life of wucai ( Brassica campestris L.)
Article Snippet: The gene‐specific primers were designed using Primer software version 5.0 (Premier Biosoft International) for the qRT‐PCR (Table ) of the genes coding for APX, AAO, DHAR, and MDHAR.

Article Title: Comparative Proteomics Reveals Cold Acclimation Machinery Through Enhanced Carbohydrate and Amino Acid Metabolism in Wucai ( Brassica Campestris L.)
Article Snippet: Specific primers for differentially expressed protein-related genes were designed by Primer software version 5.0 (Premier Biosoft International, Palo Alto, CA, USA) ( ).

Article Title: Comparative Proteomics Indicates That Redox Homeostasis Is Involved in High- and Low-Temperature Stress Tolerance in a Novel Wucai ( Brassica campestris L.) Genotype
Article Snippet: Primer software version 5.0 (Premier Biosoft International, CA, USA) was used to design gene-specific primers, and primer sequences are indicated in . qRT-PCR analysis was performed using AceQ qPCR SYBR GREEN Master Mix (Vazyme Biotechnology Co., Nanjing, China), and relative expression levels were calculated using the 2 − △△ C t method [ ].

Article Title: Mdr3 gene mutation in preterm infants with parenteral nutrition‐associated cholestasis
Article Snippet: Primer Software Version 5.0 (Premier Biosoft International) was used to design the primers, and the DNA concentration was quantified using a UV–VIS spectrophotometer (UV‐5200, Shanghai Metash Instrument Co., Ltd.).

Article Title: Cold acclimation can specifically inhibit chlorophyll biosynthesis in young leaves of Pakchoi
Article Snippet: Primers for qPCR analysis were designed by Primer Software Version 5.0 (Premier Biosoft International, CA, USA) and shown in Table S .

Article Title: Comparative Proteomic Analysis Reveals That Chlorophyll Metabolism Contributes to Leaf Color Changes in Wucai ( Brassica campestris L.) Responding to Cold Acclimation.
Article Snippet: Subscriber access provided by OCCIDENTAL COLL is published by the American Chemical Society.. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society.. Copyright © American Chemical Society.

Article Title: Comparative Proteomics Reveals Cold Acclimation Machinery Through Enhanced Carbohydrate and Amino Acid Metabolism in Wucai ( Brassica Campestris L.).
Article Snippet: Specific primers for differentially expressed protein-related genes were designed by Primer software version 5.0 (Premier Biosoft International, Palo Alto, CA, USA) (Table 3).

Expressing:

Article Title: Characterization and transcriptomic analysis of a novel yellow-green leaf wucai ( Brassica campestris L.) germplasm
Article Snippet: First-strand cDNA was synthesized using the PrimeScriptTM RT reagent kit (TaKaRa). qRT-PCR was performed in a 20-μL reaction volume containing 1 μL of 100 ng cDNA, 1 μL each of 10 μM forward and reverse primers, 10 μL SYBR Premix Ex Taq II (Takara), and filled with 7 μL ddH 2 O. Gene-specific primers were designed using Primer Software version 5.0 (Premier Biosoft International, CA, USA).

Article Title: The effect of exogenous 24‐epibrassinolide pretreatment on the quality, antioxidant capacity, and postharvest life of wucai ( Brassica campestris L.)
Article Snippet: The gene‐specific primers were designed using Primer software version 5.0 (Premier Biosoft International) for the qRT‐PCR (Table ) of the genes coding for APX, AAO, DHAR, and MDHAR.

Article Title: Comparative Proteomics Reveals Cold Acclimation Machinery Through Enhanced Carbohydrate and Amino Acid Metabolism in Wucai ( Brassica Campestris L.)
Article Snippet: Specific primers for differentially expressed protein-related genes were designed by Primer software version 5.0 (Premier Biosoft International, Palo Alto, CA, USA) ( ).

Article Title: Comparative Proteomics Indicates That Redox Homeostasis Is Involved in High- and Low-Temperature Stress Tolerance in a Novel Wucai ( Brassica campestris L.) Genotype
Article Snippet: Primer software version 5.0 (Premier Biosoft International, CA, USA) was used to design gene-specific primers, and primer sequences are indicated in . qRT-PCR analysis was performed using AceQ qPCR SYBR GREEN Master Mix (Vazyme Biotechnology Co., Nanjing, China), and relative expression levels were calculated using the 2 − △△ C t method [ ].

Article Title: Mdr3 gene mutation in preterm infants with parenteral nutrition‐associated cholestasis
Article Snippet: Primer Software Version 5.0 (Premier Biosoft International) was used to design the primers, and the DNA concentration was quantified using a UV–VIS spectrophotometer (UV‐5200, Shanghai Metash Instrument Co., Ltd.).

Article Title: Cold acclimation can specifically inhibit chlorophyll biosynthesis in young leaves of Pakchoi
Article Snippet: Primers for qPCR analysis were designed by Primer Software Version 5.0 (Premier Biosoft International, CA, USA) and shown in Table S .

Article Title: Comparative Proteomic Analysis Reveals That Chlorophyll Metabolism Contributes to Leaf Color Changes in Wucai ( Brassica campestris L.) Responding to Cold Acclimation.
Article Snippet: Subscriber access provided by OCCIDENTAL COLL is published by the American Chemical Society.. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society.. Copyright © American Chemical Society.

Article Title: Comparative Proteomics Reveals Cold Acclimation Machinery Through Enhanced Carbohydrate and Amino Acid Metabolism in Wucai ( Brassica Campestris L.).
Article Snippet: Specific primers for differentially expressed protein-related genes were designed by Primer software version 5.0 (Premier Biosoft International, Palo Alto, CA, USA) (Table 3).



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